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Cell Biology
Apoptosis Products
Apoptosis Assay Kits
APC Annexin V
APC Annexin V from BioLegend
(2)
Write a review
Citations:
(140)
Supplier Page
Supplier Page from
BioLegend for
APC Annexin V
Description
APC Annexin V
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
APC Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640920
Quantity
100 tests
Applications
Flow Cytometry (FC)
Conjugate/Tag/Label
APC
NCBI Full Gene Name
annexin A5
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (140)
(1)
In situ immunomodulation of tumors with biosynthetic bacteria promote anti-tumor immunity
Bioactive Materials
February 1, 2024
Zhongda Lin, Fanqiang Meng, Yumeng Ma, Chi Zhang, Zhirang Zhang, Zhaoxin Yang, Yuan Li, Linlin Hou, Yuzhong Xu, Xin Liang,
et al.
IL-7 for 48 h. A part of the CTLL-2 cells was stained with APC anti-Annexin V antibody (
BioLegend
,
640920
) and 7-AAD
for flow cytometry
analysis. And the rest of the CTLL-2 cells were lysed
for western blot
(2)
LFA-1 regulated by IL-2/STAT5 pathway boosts antitumor function of intratumoral CD8+ T cells for improving anti-PD-1 antibody therapy
Oncoimmunology
December 21, 2023
Jiqi Shan, Wei Jing, Yu Ping, Chunyi Shen, Dong Han, Fengsen Liu, Yaqing Liu, Congcong Li, Yi Zhang
10:1
in vitro
. After 24 h, B16
tumor
cells were collected and stained with Annexin V (
BioLegend
, cat. #
640920
) and
7-AAD Viability Staining Solution
(
BioLegend
, cat. #
420404
).
Flow cytometry
was used to analyze
(3)
Magnetic Resonance Imaging of Macrophage Response to Radiation Therapy
Cancers (Basel)
December 17, 2023
Harrison Yang, Brock Howerton, Logan Brown, Tadahide Izumi, Dennis Cheek, J. Anthony Brandon, Francesc Marti, Roberto Gedaly, Reuben Adatorwovor, Fanny Chapelin
Annexin-V buffer (Cat#
422201
,
Biolegend
) and, subsequently, stained with APC-Annexin V antibody (Cat#
640920
,
Biolegend
). Cells were washed and resuspended for flow processing. Data were acquired on a BD LSRFortessa
(4)
Tumor-repopulating cell-derived microparticles elicit cascade amplification of chemotherapy-induced antitumor immunity to boost anti-PD-1 therapy
Signal Transduction and Targeted Therapy
October 25, 2023
Nana Bie, Tuying Yong, Zhaohan Wei, Qingle Liang, Xiaoqiong Zhang, Shiyu Li, Xin Li, Jianye Li, Lu Gan, Xiangliang Yang
#304005,
clone
HI30, diluted to 1/20). The isolated
tumor
cells were stained with anti-Annexin V (catalog #
640920
) and Zombie
Violet
(catalog #
423113
) for apoptosis analysis. All antibodies applied
for flow cytometry
(5)
Combined PI3K Inhibitor and Eribulin Enhances Anti-Tumor Activity in Preclinical Models of Paclitaxel-Resistant, PIK3CA-Mutated Endometrial Cancer
Cancers (Basel)
October 8, 2023
Yeong Gyu Jeong, Nar Bahadur Katuwal, Min Sil Kang, Mithun Ghosh, Sa Deok Hong, Seong Min Park, Seul-Gi Kim, Tae Hoen Kim, Yong Wha Moon
apoptosis rate following drug treatment,
staining
was carried out using
APC annexin V
(catalog no.
640920
,
BioLegend
) and propidium iodide (PI) (catalog no. P3566, Sigma-Aldrich). After 72 h of drug treatment,
(6)
Severe fever with thrombocytopenia syndrome virus induces platelet activation and apoptosis via a reactive oxygen species-dependent pathway
Redox Biology
September 1, 2023
Yi-Hui Li, Xue-Hui Wang, Wen-Wu Huang, Ren-Rong Tian, Wei Pang, Yong-Tang Zheng
anti-mouse-CD41-PE (
clone
MWReg30); anti-
mouse
-CD62P-APC (
clone
RMP-1); anti- Annexin V-APC (
Biolegend
, #
640920
); anti-Active Caspase-3-FITC (
clone
C92-605); anti-human-CD41-Percp-Cy5.5 (
clone
HIP8); anti-human-CD62P-FITC
(7)
End-binding protein 1 regulates the metabolic fate of CD4+ T lymphoblasts and Jurkat T cells and the organization of the mitochondrial network
Frontiers in Immunology
July 13, 2023
Álvaro Gómez-Morón, Silvia Requena, Clara Pertusa, Marta Lozano-Prieto, Diego Calzada-Fraile, Camila Scagnetti, Inés Sánchez-García, Ana Adela Calero-García, Manuel Izquierdo, Noa B. Martín-Cófreces
(
Tonbo biosciences
) in 100 µl PBS during 30 min at 4°C and with 2 µl APC-conjugated annexin V (No.
640920
Biolegend
) in 100 µl
Annexin V Binding Buffer
(No.
422201
,
Biolegend
) for 15 min at RT, following the
(8)
Role of mitochondria in the myopathy of juvenile dermatomyositis and implications for skeletal muscle calcinosis
Journal of Autoimmunity
July 1, 2023
Bhargavi Duvvuri, Lauren M. Pachman, Payton Hermanson, Ting Wang, Richard Moore, Dennis Ding-Hwa Ding-Hwa Wang, Aaron Long, Gabrielle A. Morgan, Stephen Doty, Rong Tian,
et al.
analysis (
flow cytometry
) Viability analysis was done using APC-conjugated Annexin V (
Biolegend
Cat. No.
640920
and propidium iodide (
Miltenyi Biotec
Cat. No.
130–093-233
). Briefly, adhered RH30 cells following trypsinization
(9)
Chimeric antigen receptor T cells targeting FcRH5 provide robust tumour-specific responses in murine xenograft models of multiple myeloma
Nature Communications
June 20, 2023
Dongpeng Jiang, Haiwen Huang, Huimin Qin, Koukou Tang, Xiangru Shi, Tingting Zhu, Yuqing Gao, Ying Zhang, Xiaopeng Tian, Jianhong Fu,
et al.
AP1903 (MedChemExpress) for 24 h, followed by
staining
with Annexin V-APC (
Biolegend
, catalog number
640920
, dilution:
1:500
) and 7-AAD (Biolegend, catalog number 559925, dilution:
1:500
) according to the manufacturer’s
(10)
MicroRNA-708 emerges as a potential candidate to target undruggable NRAS
PLOS ONE
April 21, 2023
Jia Meng Pang, Po-Chen Chien, Ming-Chien Kao, Pei-Yun Chiu, Pin-Xu Chen, Yu-Ling Hsu, Chengyang Liu, Xiaowei Liang, Kai-Ti Lin
48 hr
transfection
. Cells were then collected and double stained with 80 ng/mL Annexin V (
BioLegend
,
640920
) and 5 μg/mL 7-AAD (
BioLegend
,
420403
) in calcium-rich binding buffer (10mM pH 7.8 HEPES, 140mM NaCl,
(11)
Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustion
Molecular cell
April 20, 2023
Battistello E, Hixon KA, Comstock DE, Collings CK, Chen X, Rodriguez Hernaez J, Lee S, Cervantes KS, Hinkley MM, Ntatsoulis K,
et al
.
IFN-γ (
BioLegend
, Cat#
505825
). Annexin
staining
was performed with
APC Annexin V
(
BioLegend
, Cat#
640920
). Samples were analyzed using a Fortessa cytometer.
Chromatin
-focused
CRISPR
screen Library design For
(12)
Expansion of circulating stem-like CD8(+) T cells by adding CD122-directed IL-2 complexes to radiation and anti-PD1 therapies in mice
Nature communications
April 12, 2023
Onyshchenko K, Luo R, Guffart E, Gaedicke S, Grosu AL, Firat E, Niedermann G.
(
eBiosciences
,
88-8824-00
) according to the manufacturer’s instructions. The APC Annexin V/PI Kit (
BioLegend
,
640920
) was used to determine the proportion of apoptotic cells. Data were acquired using a CytoFlex S Flow
(13)
Small-Molecule Cyclophilin Inhibitors Potently Reduce Platelet Procoagulant Activity
International Journal of Molecular Sciences
April 12, 2023
Jens Van Bael, Aline Vandenbulcke, Abdelhakim Ahmed-Belkacem, Jean-François Guichou, Jean-Michel Pawlotsky, Jelle Samyn, Arjan D. Barendrecht, Coen Maas, Simon F. De Meyer, Karen Vanhoorelbeke,
et al.
(HEPES)-buffered saline (pH 7.3) in the presence or absence of 2.5 mM CaCl2 and AnnexinV (APC; cat. n°
640920
,
Biolegend
, San Diego, CA, USA). Anti-
CD41
(BV421; Biolegend, San Diego, CA, USA) or anti-
CD42b
(Super
(14)
Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation
Nature communications
January 16, 2023
Liu X, Ye Y, Zhu L, Xiao X, Zhou B, Gu Y, Si H, Liang H, Liu M, Li J,
et al
.
incubated with 100 μL binding buffer (Biolegend) containing 5 μL APC-conjugated Annexin V (
1:20
, cat#
640920
,
Biolegend
) for 15 min at room temperature. After
incubation
, the cells were washed and resuspended
(15)
Phosphoproteomic profiling of T cell acute lymphoblastic leukemia reveals targetable kinases and combination treatment strategies
Nature Communications
December 1, 2022
Valentina Cordo’ et al.
staining
of apoptotic cells, 200,000 cells were stained with Annexin V-APC antibody (
Biolegend
cat#
640920
) diluted
1:20
in
Annexin V-binding buffer
(
Invitrogen
cat#
V13246
) for 15 min at room temperature (RT)
(16)
Inhibition of SRPK1, a key splicing regulator, exhibits antitumor and chemotherapeutic-sensitizing effects on extranodal NK/T-cell lymphoma cells
BMC cancer
October 27, 2022
He C, Liu B, Wang HY, Wu L, Zhao G, Huang C, Liu Y, Shan B, Liu L.
resistance.
Flow cytometry
assay
Annexin V (
420404
,
Biolegend
, USA) and 7-amino-actinomycin D (7-AAD,
640920
,
Biolegend
, USA) double
staining
was performed to detect cell apoptosis. Cells were analyzed using the
(17)
G6PD promotes cell proliferation and dexamethasone resistance in multiple myeloma via increasing anti-oxidant production and activating Wnt/β-catenin pathway
Experimental hematology & oncology
October 21, 2022
Li R, Ke M, Qi M, Han Z, Cao Y, Deng Z, Qian J, Yang Y, Gu C.
Subsequently, cell pellets were resuspended in 100 μL 1 × Binding buffer, to which 5 μL Annexin V-APC (#
640920
,
Biolegend
, Beijing, China) and 5 μL PI (50 μg/mL, #25535-16-4, Solarbio Life Science, Beijing, China)
(18)
Polyethylenimine, an Autophagy-Inducing Platinum-Carbene-Based Drug Carrier with Potent Toxicity towards Glioblastoma Cancer Stem Cells
Cancers (Basel)
October 15, 2022
Conor McCartin, Candice Dussouillez, Chloé Bernhard, Eric Mathieu, Juliette Blumberger, Monique Dontenwill, Christel Herold-Mende, Ahmed Idbaih, Philippe Lavalle, Stéphane Bellemin-Laponnaz,
et al.
140 mM NaCl, 2.5 mM CaCl2, pH 7.3–7.4). Triplicates were pooled and stained with APC-conjugated AnV (#
640920
,
BioLegend
, Amsterdam, Netherlands) diluted 1/100 with prepared AnV binding buffer which was incubated
(19)
Tumorous expression of NAC1 restrains antitumor immunity through the LDHA-mediated immune evasion
Journal for ImmunoTherapy of Cancer
September 23, 2022
Yijie Ren et al.
(St Louis, MI). The apoptosis analysis was performed using
APC Annexin V
Apoptosis Detection
Kit (#
640920
) with the Aqua Live/Dead (#
423101
) from
BioLegend
(San Diego, California, USA). Glycolysis analysis For
(20)
A Planar Culture Model of Human Absorptive Enterocytes Reveals Metformin Increases Fatty Acid Oxidation and Export
Cellular and Molecular Gastroenterology and Hepatology
April 28, 2022
Ismael Gomez-Martinez, R. Jarrett Bliton, Keith A. Breau, Michael J. Czerwinski, Ian A. Williamson, Jia Wen, John F. Rawls, Scott T. Magness
anti-
human
hashtag 6 antibody,"BioLegend (San Diego, CA)",
394641
Annexin V-APC,"
BioLegend
(San Diego, CA)",
640920
RNAqueous-Micro Total RNA Isolation Kit
,"
Thermo Fisher
(Waltham, MA)",
AM1931
"Chromium Next GE M Single-Cell
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Reviews
Write a Review
(1)
8/6/2018
Annexin V APC for Determining Apoptosis in Cells
Suzanne Tomchuck
We often look at the effects of different compounds on cell viability and apoptosis. We typically use Annexin V in combination with 7AAD or PI, to determine apoptosis by flow cytometry. We prefer the...
Read Review
(1)
2/24/2017
Staining T Cells With Annexin V APC
Adam Guess
This reagent was used to characterize living versus apoptotic/necrotic cells. It was chosen because it is the gold standard for measuring apoptosis, APC is bright and works well with other ...
Read Review
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APC Annexin V from BioLegend
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View Company Product Page
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